Vizzini: [Vizzini stops suddenly, and falls dead to the right]
Buttercup: And to think, all that time it was your cup that was poisoned.
Man in Black: They were both poisoned. I spent the last few years building up an immunity to iocane powder.
Buttercup: Hi-yo!
Full Metal Jacket (with a number of other memorably insulting quotes):
Gunnery Sergeant Hartman: How tall are you, private?
The Karate Kid:[just before Johnny fights Daniel in the tournament]
Kreese: Sweep the leg. [Johnny stares at him in shock]
Kreese: Do you have a problem with that?
Johnny: No, Sensei.
Kreese: No mercy.
Johnny: Hi-yo!
Dirty Harry:Harry Callahan: I know what you're thinking. "Did he fire six shots or only five?" Well, to tell you the truth, in all this excitement I kind of lost track myself. But being as this is a .44 Magnum, the most powerful handgun in the world, and would blow your head clean off, you've got to ask yourself a question: Do I feel lucky? Well, do ya, punk?
[other police officers show up and intervene]
Bank Robber: I gots to know.
[Harry demonstrates that there were in fact no more rounds left]
Bank Robber [shaking finger]: Hi-yo!
Rocky: Mickey: Your nose is broken.
Rocky: How does it look?
Mickey: Ah, it's an improvement.
Rocky: Hi-yo!
Braveheart:
Stephen: Stephen is my name. I'm the most wanted man on my island. Except I'm not on my island, of course. More's the pity.
Hamish: Your island? You mean Ireland.
Stephen: [Laughs] I've come to the right place then.
William & Hamish: Hi-yo!
Top Gun (this one deserves two):
Slider: Goose who's butt did you kiss to get in here anyway?
Goose: The list is long, but distinguished.
Slider: Yeah, well so is my Johnson.
Iceman: Hi-yo!
Stinger: Maverick, you just did an incredibly brave thing. What you should have done was land your plane! you don't own that plane, the tax payers do! Son, your ego is writing checks your body can't cash. You've been busted, you lost your qualifications as section leader three times, put in hack twice by me, with a history of high speed passes over five air control towers, and one admiral's daughter!
Goose: Penny Benjamin? [Maverick shrugs]
Stinger: And you asshole, you're lucky to be here!
Stinger: And let's not bullshit Maverick. Your family name ain't the best in the Navy. You need to be doing it better, and cleaner than the other guy. Now what is it with you?
Maverick: Just want to serve my country, be the best pilot in the Navy, sir.
Stinger: Don't screw around with me Maverick. You're a hell of an instinctive pilot. Maybe too good. I'd like to bust your butt but I can't. I got another problem here. I gotta send somebody from this squadron to Miramar. I gotta do something here, I still can believe it. I gotta give you your dream shot! I'm gonna send you up against the best. You two characters are going to Top Gun.
Goose & Maverick [in simultaneous fist pump]: Hi-yo!
Gladiator: Juba: Can they hear you?
Juba: Your family. In the afterlife.
Juba: What do you say to them?
Maximus: To my son - I tell him I will see him again soon. To keep his heels down while riding his horse. To my wife... that is not your business.
Juba: Hi-yo!
The Matrix:Neo: Yeah. That sounds like a really good deal. But I got a better one. How about... I give you the finger... and you give me my phone call?
Neo: You can't scare me with this Gestapo crap. I know my rights. I want my phone call.
Agent Smith: Tell me, Mr. Anderson... what good is a phone call... if you're unable to speak?
[Neo’s mouth disappears and all that can be heard are strained muffles…]
Agent Smith: Hi-yo!
And from one of my new favorite films of the moment (however not as recognizable)…Thank You for Smoking:
Brad: Nick, your job and everything aside, I hope you understand that second hand smoke's a real killer.
Brad: I just hope you're providing a smoke-free environment for Joey is all I'm saying.
Nick Naylor: Brad, I'm his *father*. You're the guy f-ing his mom.
Brad [going up for a high five but no response from Nick]: Hi-yo!